mouse gem 2.08 cdna microarray Search Results


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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional <t>lncRNA</t> (AK082424) in adult murine testes. Brown, punctuated dots representing <t>specific</t> <t>hybridization</t> signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.
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Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional lncRNA (AK082424) in adult murine testes. Brown, punctuated dots representing specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.

Journal: Biology of Reproduction

Article Title: Expression Profiling Reveals Developmentally Regulated lncRNA Repertoire in the Mouse Male Germline 1

doi: 10.1095/biolreprod.113.113308

Figure Lengend Snippet: Subcellular localization of two lncRNAs in adult murine testes by ISH assays. A–D) ISH-based localization of a bidirectional lncRNA (AK082424) in adult murine testes. Brown, punctuated dots representing specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids in seminiferous tubules at stages I–III, VII, and XI. Arrows point to representative positive cells (B–D). Sections hybridized to control probes show no specific hybridization signals (A). Insets show the digitally magnified view of the framed regions. Panels B–D are in the same magnification. Bar = 50 μm. E) Schematic of the localization of lncRNA AK082424 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are confined to the nuclei of spermatocytes and round spermatids (red dots). Roman numerals (I–XII) mark stages of the seminiferous epithelial cycle, and steps of spermatid differentiation (i.e., spermiogenesis) are labeled with Arabic numerals (1–16). Sc, Sertoli cells; As, single type A spermatogonia; As-pr, paired type A spermatogonia; Aal, aligned type A spermatogonia; A1-4, type A1-A4 spermatogonia; In, intermediate spermatogonia; B, type B spermatogonia; Pl, preleptotene spermatocyte; L, leptotene spermatocyte; Z, zygotene spermatocyte; P, pachytene spermatocyte; Di, diplotene spermatocyte; M, meiotically dividing. F–I) ISH-based localization of an antisense lncRNA (AK016507) in adult mouse testes. Brown, punctuated dots representing specific hybridization signals are confined mainly to the cytoplasm of spermatocytes and round and elongating spermatids in seminiferous tubules at stages IV, V, VII, X, and XI. Arrows point to representative positive cells (G–I). Sections hybridized to control probes show no specific hybridization signals (F). Insets show the magnified views of the framed regions. Panels F–I are all in the same magnification. Bar = 50 μm. J) Schematic of localization of lncRNA AK016507 during spermatogenesis in adult murine testes. Cells expressing this lncRNA are framed, and the height of the frame represents relative expression levels. Note that specific hybridization signals are mainly confined to the cytoplasm of spermatocytes and round and elongating spermatids (red dots). Labels are the same as in E.

Article Snippet: The labeled cDNAs were hybridized to the Mouse LncRNA Array (version 2.0; 8 × 60 K; Arraystar) using the SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent).

Techniques: Hybridization, Control, Expressing, Labeling